Icariin Powder
Description
Plant Horny Goat Weed commonly known as epimedium and yinyanghuo.Icariin 98% cas 489-32-7 is a major flavonol glycoside from Horny Goat Weed Extract. Purified icariin powder is used to icariin supplements.
Secification:
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Chemical Name |
4′-O-methyl-8-gamma,γ-dimethylallylkaempferol-3-rhamnoside-7-glucoside |
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Purity |
98% (Iidentified by HPLC;MS;NMR) |
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CAS No |
489-32-7 |
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Chemical family |
Flavonoids |
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Appearance |
Yellow powder |
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Botanical sources |
Aerial part of Epimedium sagitattum |
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Solubility |
Icariin powder is good soluble in organic solvents methanol, DMSO and dimethy formamide. |
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Storage |
Store in a cool place,closed container, protected from air and light. |
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Packaging |
1 g , 10g in bottle glass, 1kg in bag |
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Delivery time |
2-3 days Upon confirmed order. Shipped via Fedex,DHL.EMS ,TNT . |
High performance liquid chromatography method for Icariin
Chromatographic system
C18 Column ,Wavelength with 270nm
Mobile Phase is a Acetonitrile and water (30:70,V/V)
Icariin Standard solution.
Weight accurately icariin and dissolve in Methanol.Dilute with Methanol to produce solution of 0.1mg/ml for icariin.
Icariin sample solution.
Weigh accurately 0.5 g icariin powder sample and place it in a 50-mL centrifuge tube, then add 10 mL of ethanol (50%). Sonicate (300 W) the mixture for 30 min. Centrifuge at about 2000 × g for 10 min. Transfer the supernatant to a 25-mL volumetric flask. Repeat the extraction for two more times each with 7 mL of ethanol (50%). Combine the supernatants and make up to the mark with ethanol (50%). Filter through a 0.45-µm nylon filter.
Procedure
Inject icariin Standard solution and icariin sample solution (10μl each) into the HPLC system and record the chromatograms,Measure the peak area and calsulate the icariin concentration.
